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domain ecd protein  (Sino Biological)


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    Structured Review

    Sino Biological domain ecd protein
    Domain Ecd Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+lag3+ecd/Human+LAG3+%2F+CD223+%2F+Lymphocyte+activation+gene+3+Protein/pm41016188-147-36-42
    Average 94 stars, based on 15 article reviews
    domain ecd protein - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Antibodies binding LAG3 and methods of treatment using them
    Article Snippet: .. Screening of Hybridoma Cell Lines by ELISA High-throughput ELISA binding assay was firstly used to screen for hybridoma clones producing monoclonal antibodies binding to human LAG3(ECD)-his (Sino Biological, CN, Cat #:16498-H08H). .. The clones producing human LAG3 binders were further tested for their abilities to cross-react with monkey or mouse LAG3, using rhesus LAG3(ECD)-hFc (ACRO, CN, Cat #: LA3-05252) and mouse LAG3(ECD)-his (Sino Biological, CN, Cat #:53069-M08H).

    Article Title: Antibodies binding LAG3 and methods of treatment using them
    Article Snippet: The human LAG3(ECD)-hFc and monkey LAG3(ECD)-hFc proteins were emulsified by sonication with an equal volume of Complete Freund's Adjuvant (SIGMA, USA, Cat #: F5881-10*10ML), Incomplete Freund's Adjuvant (SIGMA, USA, Cat #: F5506-6*10ML), or PBS. .. TABLE 2 Immunization scheme Primary 1st Boost 2nd Boost 3rd Boost Final Boost Day 0 14 28 42 56 Protein Human Human rh Human rh LAG3 and dose LAG3(ECD)- LAG3(ECD)- LAG3(ECD)- LAG3(ECD)- (ECD)-hFc hFc hFc hFc hFc (25 ug/ (50 μg/ (50 μg/ (50 μg/ (50 μg/ mouse) + mouse) mouse) mouse) mouse) Human LAG3 (ECD)-hFc (25 μg/mouse) Adjuvant Complete Incomplete Incomplete Incomplete PBS Freund's Freund's Freund's Freund's Way of i.p. i.p. i.p. i.p. i.v. immunization One week after each boost, 50 μl of murine serum was collected from each mouse for titer determination by ELISA using the recombinant human LAG3(ECD)-his (Sino Biological, CN, Cat #:16498-H08H), monkey LAG3(ECD)-hFc (ACRO, CN, Cat #: LA3-05252), and mouse LAG3(ECD)-his (Sino Biological, CN, Cat #: 53069-M08H). ..

    Article Title: Antibodies binding LAG3 and methods of treatment using them
    Article Snippet: .. ELISA plates were coated with 50 μl of 500 ng/ml human LAG3(ECD)-his (Sino Biological, CN, Cat #:16498-H08H) at 4° C. overnight. ..

    Binding Assay:

    Article Title: Antibodies binding LAG3 and methods of treatment using them
    Article Snippet: .. Screening of Hybridoma Cell Lines by ELISA High-throughput ELISA binding assay was firstly used to screen for hybridoma clones producing monoclonal antibodies binding to human LAG3(ECD)-his (Sino Biological, CN, Cat #:16498-H08H). .. The clones producing human LAG3 binders were further tested for their abilities to cross-react with monkey or mouse LAG3, using rhesus LAG3(ECD)-hFc (ACRO, CN, Cat #: LA3-05252) and mouse LAG3(ECD)-his (Sino Biological, CN, Cat #:53069-M08H).

    Clone Assay:

    Article Title: Antibodies binding LAG3 and methods of treatment using them
    Article Snippet: .. Screening of Hybridoma Cell Lines by ELISA High-throughput ELISA binding assay was firstly used to screen for hybridoma clones producing monoclonal antibodies binding to human LAG3(ECD)-his (Sino Biological, CN, Cat #:16498-H08H). .. The clones producing human LAG3 binders were further tested for their abilities to cross-react with monkey or mouse LAG3, using rhesus LAG3(ECD)-hFc (ACRO, CN, Cat #: LA3-05252) and mouse LAG3(ECD)-his (Sino Biological, CN, Cat #:53069-M08H).

    Bioprocessing:

    Article Title: Antibodies binding LAG3 and methods of treatment using them
    Article Snippet: .. Screening of Hybridoma Cell Lines by ELISA High-throughput ELISA binding assay was firstly used to screen for hybridoma clones producing monoclonal antibodies binding to human LAG3(ECD)-his (Sino Biological, CN, Cat #:16498-H08H). .. The clones producing human LAG3 binders were further tested for their abilities to cross-react with monkey or mouse LAG3, using rhesus LAG3(ECD)-hFc (ACRO, CN, Cat #: LA3-05252) and mouse LAG3(ECD)-his (Sino Biological, CN, Cat #:53069-M08H).

    Incubation:

    Article Title: Antibodies binding LAG3 and methods of treatment using them
    Article Snippet: .. Human LAG3(ECD)-his (Sino Biological, CN, Cat #:16498-H08H), 0.5 μg/mL, 50 μl per well, was added to the plates which were further incubated for 1 hour at RT. ..

    Adjuvant:

    Article Title: Antibodies binding LAG3 and methods of treatment using them
    Article Snippet: The human LAG3(ECD)-hFc and monkey LAG3(ECD)-hFc proteins were emulsified by sonication with an equal volume of Complete Freund's Adjuvant (SIGMA, USA, Cat #: F5881-10*10ML), Incomplete Freund's Adjuvant (SIGMA, USA, Cat #: F5506-6*10ML), or PBS. .. TABLE 2 Immunization scheme Primary 1st Boost 2nd Boost 3rd Boost Final Boost Day 0 14 28 42 56 Protein Human Human rh Human rh LAG3 and dose LAG3(ECD)- LAG3(ECD)- LAG3(ECD)- LAG3(ECD)- (ECD)-hFc hFc hFc hFc hFc (25 ug/ (50 μg/ (50 μg/ (50 μg/ (50 μg/ mouse) + mouse) mouse) mouse) mouse) Human LAG3 (ECD)-hFc (25 μg/mouse) Adjuvant Complete Incomplete Incomplete Incomplete PBS Freund's Freund's Freund's Freund's Way of i.p. i.p. i.p. i.p. i.v. immunization One week after each boost, 50 μl of murine serum was collected from each mouse for titer determination by ELISA using the recombinant human LAG3(ECD)-his (Sino Biological, CN, Cat #:16498-H08H), monkey LAG3(ECD)-hFc (ACRO, CN, Cat #: LA3-05252), and mouse LAG3(ECD)-his (Sino Biological, CN, Cat #: 53069-M08H). ..

    Recombinant:

    Article Title: Antibodies binding LAG3 and methods of treatment using them
    Article Snippet: The human LAG3(ECD)-hFc and monkey LAG3(ECD)-hFc proteins were emulsified by sonication with an equal volume of Complete Freund's Adjuvant (SIGMA, USA, Cat #: F5881-10*10ML), Incomplete Freund's Adjuvant (SIGMA, USA, Cat #: F5506-6*10ML), or PBS. .. TABLE 2 Immunization scheme Primary 1st Boost 2nd Boost 3rd Boost Final Boost Day 0 14 28 42 56 Protein Human Human rh Human rh LAG3 and dose LAG3(ECD)- LAG3(ECD)- LAG3(ECD)- LAG3(ECD)- (ECD)-hFc hFc hFc hFc hFc (25 ug/ (50 μg/ (50 μg/ (50 μg/ (50 μg/ mouse) + mouse) mouse) mouse) mouse) Human LAG3 (ECD)-hFc (25 μg/mouse) Adjuvant Complete Incomplete Incomplete Incomplete PBS Freund's Freund's Freund's Freund's Way of i.p. i.p. i.p. i.p. i.v. immunization One week after each boost, 50 μl of murine serum was collected from each mouse for titer determination by ELISA using the recombinant human LAG3(ECD)-his (Sino Biological, CN, Cat #:16498-H08H), monkey LAG3(ECD)-hFc (ACRO, CN, Cat #: LA3-05252), and mouse LAG3(ECD)-his (Sino Biological, CN, Cat #: 53069-M08H). ..



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